Journal: International Journal of Molecular Sciences

Article Title: Mutated CCDC51 Coding for a Mitochondrial Protein, MITOK Is a Candidate Gene Defect for Autosomal Recessive Rod-Cone Dystrophy

doi: 10.3390/ijms22157875

Figure Lengend Snippet: Schematic drawing of the genomic structure and expression of CCDC51 . ( a ) CCDC51 is located on chromosome 3 and exons 2–4 code for a 411 amino acid protein. Unfilled and filled boxes represent untranslated and translated regions, respectively. The arrow depicts the translation start. M represents the identified mutation. ( b ) The four exons of CCDC51 are expressed in normal human retina (red) . ( c ) CCDC51 RT-PCR encompassing exons 2–4 revealed the presence of the transcript in all tested tissues and cells including human retina, human universal tissues control cDNA, human fibroblasts, human blood, COS-1, and HeLa cells shown on an agarose gel with an expected size of ~660 bp.

Article Snippet: Expression of the candidate gene has been experimentally validated in human retina (Clonetech, Palo Alto, California), in human control cDNA (universal, multi-tissues, Clonetech), in human fibroblast, in blood, in COS-1 and HeLa cells with primers in exon 2 and 4 of CCDC51 (Ex2cDNAF: 5′-CACAGCATTCAGCAACGAGC-3′ and Ex4aR: 5′-GGACTACCTGCCTGTGACC-3′), and the sequence of the transcripts was validated by Sanger sequencing with the same primers (detailed conditions will be delivered on request).

Techniques: Expressing, Mutagenesis, Reverse Transcription Polymerase Chain Reaction, Agarose Gel Electrophoresis

Journal: Scientific Reports

Article Title: A complex of novel protease inhibitor, ovostatin homolog, with its cognate proteases in immature mice uterine luminal fluid

doi: 10.1038/s41598-019-41426-4

Figure Lengend Snippet: Tissue distribution of mOH mRNA. Total RNAs isolated from various adult mice tissues or from different stages of mice embryos were subjected to RT-PCR. The resulting products were separated in agarose gel and visualized by ethidium bromide staining under exposure of UV light. The RT-PCR product of GAPDH was served as the internal control. The original agarose gels were shown in Supplementary Fig. .

Article Snippet: The multi-tissue mRNA panel of mouse was obtained from Clontech Laboratories, Inc. (Mountain View, CA, USA).

Techniques: Isolation, Reverse Transcription Polymerase Chain Reaction, Agarose Gel Electrophoresis, Staining, Control